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| The Gandel Charitable Trust Sequencing Centre |
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Denaturing High Performance Liquid Chromatography (DHPLC) The Gandel Charitable Trust Sequencing Centre was established in 1999. Several complementary services have since been introduced, including a Denaturing High Performance Liquid Chromatography (DHPLC) service in 2004 for researchers requiring analysis of genetic variation. This service is available to researchers and clinicians within the Monash Health Research Precinct (MHRP) as well as external clients from other Institutes
The Centre utilises the VARIAN Helix™ System for DHPLC analysis. DHPLC is a highly sensitive method for detecting single nucleotide polymorphisms (SNPs), mutations, and other DNA sequence variations. DNA sequence variant detection by DHPLC involves the resolution of PCR products by ion-pair reverse phase chromatography through a unique DNA separation matrix under partial denaturation conditions. DNA variations are detected as a pattern of peaks that discriminate variant double-stranded DNA from wild type DNA. Applications for DHPLC technology include: - Screening patients for disease causing mutations - Linkage analysis in humans and mice - Genotyping Enquiries for use of the DHPLC service can be made by contacting the Centre. |
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DHPLC  Helix System technology & theory [pdf] VARIAN Helix system DHPLC melt program DHPLC publications [pdf] DHPLC Sample Submission [pdf] Request form [pdf] |
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