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| The Gandel Charitable Trust Sequencing Centre |
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Real-Time PCR The Gandel Charitable Trust Sequencing Centre was established in 1999. Several complementary services have since been introduced, including a Real-Time PCR service in 2006. This service is available to researchers and clinicians within the Monash Health Research Precinct (MHRP) as well as external clients from other Institutes.
The Centre utilises the Applied Biosystems 7900HT Fast Real-Time PCR system. This is a high throughput PCR instrument equipped with Fast 96- and 384- sample blocks, as well as a Taqman® Low Density Array (TLDA) block. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs). The service offers access to the 7900HT instrument as well as the accompanying Taqman® and SYBR Green Dye assays. A complete line of reagents and consumables are available for purchase at cost price. TLDA plates, however, must be ordered directly from Applied Biosystems by the user. Use of the 7900HT instrument is arranged by a booking system. Enquiries and booking requests can be made by contacting the Centre. A Corbett Research CAS1200 liquid handling instrument is also available for Real-Time plate set up or to assist with associated projects. |
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Real-Time PCR  AB 7900HT Fast Real-Time PCR system 7900 seminar [pdf] RT PCR chemistry guide [pdf] TLDA getting started guide [pdf] AB information links AB Taqman & SNP costs [pdf] |
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