Denaturing High Performance Liquid Chromatography (dHPLC)

The Centre uses the VARIAN Helix™ System for dHPLC analysis for researchers requiring analysis of genetic variation.  dHPLC is a highly sensitive method for detecting single nucleotide polymorphisms (SNPs), mutations, and other DNA sequence variations. DNA sequence variant detection by dHPLC involves the resolution of PCR products by ion-pair reverse phase chromatography through a unique DNA separation matrix under partial denaturation conditions. DNA variations are detected as a pattern of peaks that discriminate variant double-stranded DNA from wild type DNA.

Applications for DHPLC technology include:

• Screening patients for disease causing mutations
• Linkage analysis in humans and mice
• Genotyping

Please contact the Centre for dHPLC service enquiries